Plasmid drawing software online

Please Mouse over the image objects for descriptions. Get a Free Trial. Ask a Question. Generate Quote. Overview Features Resources Draw Exceptional Publication Quality Plasmid Maps Whether you are a catalog company selling engineered plasmids or are performing restriction analysis for recombinant cloning experiments, SimVector will help you simulate the experiments and create publication quality plasmid maps from start to finish.

Live Chat by Comm In addition to designing your vectors, you can order custom cloning of your designed vectors along with related services such as virus packaging and plasmid DNA preparation from our platform. You can start designing your own vectors by clicking on Design My Vectors from our homepage , which will allow you to select the appropriate vector system suitable for your experiment from close to available systems:.

Application — Our rich vector collection includes vectors for a variety of applications, such as:. Species — We offer vectors for transgene expression in a variety of species including mammals, zebrafish, Drosophila, plants, bacteria, yeast and insects.

Once you have selected the appropriate vector system for your experiment, you can enter the Vector Design Studio to design your vectors with a variety of customization options such as:.

There are three ways to add the vector components: choosing from our popular vector components collections, searching by gene of interest GOI , or directly pasting the sequence.

The design studio allows you to place up to 4 ORFs behind a promoter as a polycistron. After you have added an ORF to your vector, you can open the ORF editor to add epitope tags or introduce mutations to your sequence. We provide dozens of popular protein tags that can be appended to either the N or C terminus of your ORF. Other handy functions including codon optimization, translating nucleotide sequence into amino acid sequence, finding ORFs, and viewing the reverse-complementary strand are available.

After you have finished designing your vector, you will be redirected to the Vector Information page. From here you can:. You can use this ID to retrieve full information about the vector through the Retrieve Vector Information under menu item Design Vector without logging in. Our highly experienced scientists will design your vector for free and send you the design along with price and turnaround information.

In addition to designing your own vectors or sending us design requests, you can also search for popular vector designs for your GOI s by clicking on Find Popular Vectors from the VectorBuilder homepage.

This option also allows you to check if any pre-made vectors for your GOI s are available in our inventory. You can then select the vector of your choice to view its fully annotated map and sequence, and make further modifications to the design if required. Promoter — The promoter is a critical component of all expression vectors since it drives transcription of the GOI lying downstream of it.

The selection of the promoter for a vector depends on the desired location and level of target gene expression. Promoters can be either ubiquitous, driving gene expression in all cell types, or they can be tissue-specific, driving gene expression only in specific cell types.

Additionally, promoters can be of variable strengths weak, medium or strong based on the level of target gene expression that can be achieved by them. The ORF is vital for expression vectors and is placed downstream of the promoter. When multiple proteins are needed to be expressed simultaneously, ORFs corresponding to those proteins can be placed under the same promoter separated by linkers to be expressed as a polycistron.

Incorporating the polyA signal in a vector results in polyadenylation of the mRNA which protects the mRNA from being degraded by nucleases and phosphatases.

Additionally, polyadenylation is essential for the nuclear export of the mRNA into the cytosol and the translation of the protein by ribosomes. Selection marker — A selection marker enables the experimenter to successfully identify cells that have been positively transfected or transduced with the vector.

Drug-selection markers function by conferring resistance against the respective antibiotic in cells carrying the vector with the marker.

Cells not carrying the vector on the other hand are non-resistant to the antibiotic and are therefore, killed in its presence. Origin of replication — The origin of replication is essential for the replication of plasmid vectors inside bacterial host cells. The choice of the origin of replication typically depends on the number of copies of the vector that are needed to be maintained within the host cells, which in turn influences the stability of the vector.

While high-copy number plasmids help to obtain higher DNA yields from plasmid preps, low copy-number plasmids are the preferred choice for expressing toxic or insoluble proteins. Additionally, when growing more than one plasmid within the same bacterial cells it is important that both plasmids do not contain the same origin of replication since then they would be incompatible and would prevent the plasmids from replicating.

Antibiotic resistance gene — The antibiotic resistance gene allows the selection of bacterial cells carrying the vector, thereby facilitating the maintenance of the vector in the bacterial population. Cells not carrying the vector are non-resistant to the antibiotic and are therefore, killed in its presence. Other components — While the components listed above are typically found in majority of vector types, a variety of other vector components can be found in certain vector types depending on their specific application.

For example, viral vectors such as lentivirus and AAV vectors are characterized by the presence of viral sequences such as long terminal repeats LTRs and inverted terminal repeats ITRs , respectively which are essential for virus-dependent expression of the GOI s in target cells. Try for Free. Zooming for chromosomes. Primer binding sites. Enzyme sites and properties. Sequence trace viewer. Intuitive sequence editing. Realistic agarose gels. Customized enzyme sets. Get a Free Trial.

Ask a Question. Generate Quote. Overview Features Resources Draw Exceptional Publication Quality Plasmid Maps Whether you are a catalog company selling engineered plasmids or are performing restriction analysis for recombinant cloning experiments, SimVector will help you simulate the experiments and create publication quality plasmid maps from start to finish.

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